The choice of conditions for cathepsin D activity determination in human saliva
Roszkowska-Jakimiec
W, D¹browska E, Gacko M, Karwowska A, Chlabicz M
Abstract
The
aim of the study was the demonstration and choice of conditions for
the determination of cathepsin D activity in human mixed saliva. The
6% solution of hemoglobin, denatured with hydrochloric acid, was used
as the substrate. The ratio of saliva volume to hemoglobin was 4:1
w/v. The reaction was interrupted by adding 10% trichloroacetic acid,
after 6 hours of incubation at 37°C. The increase in degradation
products was determined with the use of Folina and Ciocalteau method
with copper modification.
Key words: human saliva, cathepsin
D, method of determination, inhibitor from Vicia sativa L.